Purpose: The purpose of the study was the effect of unilateral practice on leg lateral asymmetry in soccer kicking of the male students at Ferdowsi University of Mashhad. Method:The subjects were 40 students (18 to 24) who participated in the course of General Physical Education and did not have any professional and regular experiences in soccer, and their preferred leg was right. They were randomly and equally assigned to experimental and control groups. Experimental group practiced with their non-preferred left leg according to the program for eight weeks and three times a week in a 1.5 hour session. Control group had no activities other than those usually performed in their daily living duties. The subjects performed the task of instep kicking in pre-test, post-tests and retention test 15 days following the end of practice. The data were analyzed through analysis of variance tests and T test in experimental and control groups. Results:Results showed that the unilateral practice has been caused remarkable increase in the left leg performance and inconsiderably increase in the right leg performance. Also, significant reduction in the index of lateral asymmetry from the pre-test to post-test (p=0.002) and retention test (p=0.003) showed reduction of right leg preference in experimental group after the unilateral practice. Discussion:Based on the results, it is suggested that leg preference is task-specific and it is as a result of special limitations in acquisition of a task and basically comes from previous experiences of differential use of the limbs
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